ABSTRACT
Introduction: The spleen is a lymphoid organ that plays an important role in the body's defense against organisms, participation in blood filtration process, phagocytosis and immunoglobulin production. When splenectomy due to severe splenic trauma or hematological disorder is inevitable, spleen autotransplantation may be the only option to preserve the spleen. It has not been determined the structural rearrangements after transplanting in comparison with the amount of transplanted spleen. The aim of this study was to evaluate the histological changes of spleen autotransplanted in the omentum of rats.Materials and Methods: In this experimental study, 16 male Wistar rats underwent splenectomy and transplanting three pieces of splenic tissue consists of 10-15% of the greater omentum. The rats were divided to two equal groups; Eightcaseswere randomly separated and followedfor 6 months (Group Au-6) and 8 other cases also were separated for 12months'follow-up (group Au-12). At the end of follow-up period, after the re-operation ofautotransplanted rats, the splenictissues underwent the macroscopic and microscopic examination and two groups were compared together.Results: After laparotomy, splenic tissues were detected in all cases (8/8, 100%) of Au-6 group and 7 cases (87.5%) of Au-12 group. It’s observed no significant difference between two groups in the capsule around splenic tissue, organized structure, fibrosis and revascularization (P>0.05).The hemosiderinpigmentation was significantly higher in Au-12 group (P=0.03).Conclusions: The results of this study showed that autotransplanting about 15 percent of splenic tissuewere associated with a high success rate in tissue structure rearrangement.Therefore, spleen autotransplantation even in small sizes was highly recommended in cases of unavoidable splenectomy
ABSTRACT
Em órgãos potencialmente importantes na resposta imune, como o baço, alternativas como o autoimplante de segmentos esplênicos, quando a esplenectomia total torna-se necessária, e a utilização de nutrientes com funcionalidade imunomoduladora vêm sendo estudadas, objetivando minimizar o efeito pró-inflamatório persistente da sepse abdominal. O objetivo deste trabalho foi avaliar o efeito do consumo de óleo de peixe na modulação da resposta inflamatória em animais submetidos a esplenectomia total isolada ou combinada com autoimplante esplênico e à indução de sepse abdominal, verificando a possível otimização na resposta pró-inflamatória e a regeneração funcional do autoimplante. Utilizamos 64 ratos machos da linhagem Wistar, com peso variando entre 140-200 g, aleatoriamente distribuídos em oito grupos: quatro grupos-controle (100% óleo de soja) e quatro grupos-intervenção (35% de óleo de peixe), cada um com oito animais. Os dos grupos-controle (animais alimentados com ração purificada, segundo AIN-93, com conteúdo lipídico constituído por 100% óleo de soja) foram: I sem intervenção cirúrgica e, 16 semanas após, submetidos à indução de sepse abdominal; II esplenectomia total isolada e, 16 semanas após, submetidos à indução de sepse abdominal; III esplenectomia total combinada com autoimplante esplênico e, 16 semanas após, submetidos à indução de sepse abdominal; e IV esplenectomia total combinada com autoimplante esplênico e, oito semanas após, submetidos à indução de sepse abdominal. Os dos grupos-intervenção (V a VIII) foram submetidos a procedimentos similares aos executados nos grupos I a IV, respectivamente, sendo a única modificação fundamentada na substituição de 35% do conteúdo lipídico da alimentação dos animais por óleo de peixe. Todos os animais foram submetidos a sepse induzida por ligadura e perfuração cecal (CLP). Coletamos amostras sanguíneas de todos os animais antes da indução da sepse (período 1) e 2 e 4 horas (períodos 2 e 3...
In organs potentially important in the immune response, like the spleen, alternatives such as autotransplantation of splenic segments, when the total splenectomy becomes necessary, and the use of nutrients with immunomodulatory function have been studied, trying to minimize the effect of pro-inflammatory persistent abdominal sepsis. The aim of this study was to evaluate the effect of consumption of fish oil in modulating the inflammatory response in animals submitted to total splenectomy alone or combined with spleen autotransplantation and the induction of sepsis, verifying the possible optimization in the proinflammatory response and the functional regeneration of the autotransplant. We used 64 male Wistar rats, weighing between 140-200 g, were randomly distributed into eight groups: four control-groups (100% soybean oil) and four intervention-groups (35% fish oil), each one with ten animals. The rats in control groups (animals fed with purified according to the AIN-93 with lipid content consisting of 100% soybean oil) : I without surgical intervention, and 16 weeks after, submitted to the induction of abdominal sepsis II total spleenectomy alone, and 16 weeks after, submitted to the induction of abdominal sepsis; III total splenectomy combined with spleen autotransplantation, and 16 weeks after, submitted to the induction of abdominal sepsis; and IV total splenectomy combined with spleen autotransplantation, and 8 weeks after, submitted to the induction of abdominal sepsis. The rats in intervention groups (V to VIII) were subjected to similar procedures performed in groups I to IV, respectively, being the only modification based on the substitution of 35% of the lipid content of animal feed for fish oil. All animals were subjected to sepsis induced by cecal ligation and puncture (CLP). We collected blood samples from all animals before the induction of sepsis (period 1) and 2 and 4 hours (periods 2 and 3) after the induction of abdominal...